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Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in goblet cells and enterocytes.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in goblet cells and enterocytes.
Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in goblet cells and enterocytes.

Anti-GBA2 Antibody

HPA024026
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGBA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GBA2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID57704
  • Target name
    GBA2
  • Target description
    glucosylceramidase beta 2
  • Target synonyms
    AD035, NLGase, SPG46, non-lysosomal glucosylceramidase, beta-glucocerebrosidase 2, bile acid beta-glucosidase GBA2, bile acid glucosyl transferase GBA2, cholesterol glucosyltransferase GBA2, cholesteryl-beta-glucosidase GBA2, glucocerebrosidase 2, glucosidase, beta (bile acid) 2, glucosylceramidase 2, non-lysosomal cholesterol glycosyltransferase, non-lysosomal galactosylceramidase, non-lysosomal glycosylceramidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9HCG7
  • Protein Name
    Non-lysosomal glucosylceramidase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of GBA2 using anti-GBA2 antibody (A08047-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBA2 antigen affinity purified polyclonal antibody (Catalog # A08047-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBA2 at approximately 105 kDa. The expected band size for GBA2 is at 105 kDa.
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