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Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.

Anti-GBF1 Antibody

HPA037760
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGBF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GBF1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8729
  • Target name
    GBF1
  • Target description
    golgi brefeldin A resistant guanine nucleotide exchange factor 1
  • Target synonyms
    ARF1GEF, CMT2GG, CMTDI2, CMTDIA, Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1, BFA-resistant GEF 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92538
  • Protein Name
    Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GBF1 using anti-GBF1 antibody (A01817-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GBF1 antigen affinity purified polyclonal antibody (Catalog # A01817-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GBF1 at approximately 250 kDa. The expected band size for GBF1 is at 207 kDa.
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Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Product group Antibodies
Atlas Antibodies
Anti-GBF1 AntibodyHPA037759
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ReactivityHuman
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Immunohistochemical staining of human heart muscle shows cytoplasmic positivity in myocytes.
Product group Antibodies
Atlas Antibodies
Anti-GBF1 AntibodyHPA037759
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetGBF1
  • SizePrice