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Immunofluorescent staining of human cell line Hep G2 shows localization to intermediate filaments.
Immunofluorescent staining of human cell line Hep G2 shows localization to intermediate filaments.
Immunofluorescent staining of human cell line Hep G2 shows localization to intermediate filaments.

Anti-GCKR Antibody

HPA064305
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
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Product group Antibodies
TargetGCKR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GCKR
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2646
  • Target name
    GCKR
  • Target description
    glucokinase regulator
  • Target synonyms
    FGQTL5; GKRP; glucokinase (hexokinase 4) regulator; glucokinase regulatory protein; hexokinase 4 regulator
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.
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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & cytosol.
Product group Antibodies
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Figure 1. Western blot analysis of GCKR using anti-GCKR antibody (A02122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCKR antigen affinity purified polyclonal antibody (Catalog # A02122-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GCKR at approximately 69 kDa. The expected band size for GCKR is at 69 kDa.
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