Anti-GCLC Antibody [JA08-03]

HUABIO

Anti-GCLC Antibody [JA08-03]

7

Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

WB,IHC-P,FC

Research Use Only

Monoclonal

JA08-03

1 mg/ml

Unconjugated

GCLC

glutamate-cysteine ligase catalytic subunit

CNSHA7, GCL, GCS, GLCL, GLCLC, glutamate--cysteine ligase catalytic subunit, GCS heavy chain, gamma-ECS, gamma-glutamylcysteine synthetase

Rabbit

IgG

Glutamate--cysteine ligase catalytic subunit

The GCLC gene consists of 16 exons and encodes the 636 amino acid protein g-GCSc (g-glutamylcysteine synthetase heavy subunit), also designated g-L-glutamate-L-cysteine ligase catalytic subunit (GLCLC). g-GCSc is expressed in hemocytes, brain, liver and kidney. g-GCSc associates with a regulatory or modifier subunit, g-GCSm (g-glutamylcysteine synthetase light subunit), to form a heterodimer, g-GCS. g-GCS is the first enzyme involved and the rate determining step in glutathione biosynthesis. Oxidants, cadium and methyl mercury upregulate the transcription of g-GCS. H2O2 regulation depends on the Yap1 protein and the presence of glutamate, glutamine and lysine. Cadium regulates transcription through proteins Met-4, Met-31 and Met-32. Cbf1, a DNA binding protein, inhibits transcription of g-GCS. Chemopreventive compounds cause increased levels of g-GCSc in kidney tissues, which may protect against chemically induced carcinogenesis. A His370Leu amino acid change in g-GCSc causes deficiencies in activity which are responsible for hemolytic anemia and low red blood cell glutathione levels.

Human, Rat

Human,Rat

-20°C,2°C to 8°C

41116161