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Anti-GDF15 Antibody

Anti-GDF15 Antibody

A101082
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-GDF15 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to GDF15.
  • Reactivity
    Human
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GDF15 using anti-GDF15 antibody (A01583-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 22RV whole Cell lysates, Lane 2: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GDF15 antigen affinity purified polyclonal antibody (Catalog # A01583-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GDF15 at approximately 34KD. The expected band size for GDF15 is at 34KD.
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Lane 1: Mouse Urinary bladder lysates; Lane 2: Mouse Large intestine lysates; Lane 3: Rat Urinary bladder lysates; Lane 4: Rat Large intestine lysates; Lane 5: Human HT1080 cell lysates; Lane 6: Human HepG2 cell lysates; Lane 7: Human K562 cell lysates; Lane 8: Human A549 cell lysates probed with GDF15/MIC-1 Polyclonal Antibody, Unconjugated (bs-3818R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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