Anti-Genome polyprotein [PVSP19B], Camelid VHH, His-tagged,

Absolute Antibody

Anti-Genome polyprotein [PVSP19B], Camelid VHH, His-tagged,

9

This antibody is specific for Capsid proteins VP1 (GH loop, EF loop and C beta strand) and VP2 (EF loop) of the polio genome polyprotein.

The binding strength of the original antibody to poliovirus antigens was determined using surface plasmon resonance (Thys et al., 2011; PMID:21371506). The original antibody was used for a neutralization assay against three types of poliovirus. This antibody showed strong neutralization in type 1 polioviruses. The cytotoxicity of this antibody on HeLa cells was also evaluated. It showed no cytotoxicity (Thys et al., 2010; PMID:20566349). The effect of this antibody on virus entry and uncoating was determined. It showed change in the behavior of the polio particles (Schotte et al., 2014; PMID:24501405). The structure of this antibody was determined using cryo-EM (Strauss et al., 2016; PMID:26764003).

Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

PVSP19B

PVgp1

capsid protein VP0;genome polyprotein;precursor polypeptide P1;precursor polypeptide P2;precursor polypeptide P3;precursor protein 3BCD;protein 2BC

capsid protein VP0;genome polyprotein;precursor polypeptide P1;precursor polypeptide P2;precursor polypeptide P3;precursor protein 3BCD;protein 2BC; PVgp1

Camel

Virus

-20°C,2°C to 8°C

12352203