Anti-Genome polyprotein [PVSS21E], Camelid VHH, His-tagged,

Absolute Antibody

Anti-Genome polyprotein [PVSS21E], Camelid VHH, His-tagged,

9

The binding strength of the original antibody to poliovirus antigens was determined using surface plasmon resonance (Thys et al., 2011; PMID:21371506). The original antibody was used for a neutralization assay against three types of poliovirus. This antibody showed strong neutralization in type 1 polioviruses. The cytotoxicity of this antibody on HeLa cells was also evaluated. It showed no cytotoxicity (Thys et al., 2010; PMID:20566349). The effect of this antibody on virus entry and uncoating was determined. It showed change in the behavior of the polio particles (Schotte et al., 2014; PMID:24501405). The structure of this antibody was determined using cryo-EM (Strauss et al., 2016; PMID:26764003).

Neutralisation/Blocking, Other Application

Research Use Only

Monoclonal

PVSS21E

PVgp1

capsid protein VP0;genome polyprotein;precursor polypeptide P1;precursor polypeptide P2;precursor polypeptide P3;precursor protein 3BCD;protein 2BC

PVgp1, capsid protein VP0;genome polyprotein;precursor polypeptide P1;precursor polypeptide P2;precursor polypeptide P3;precursor protein 3BCD;protein 2BC, RNA-directed RNA polymerase, capsid protein VP1, capsid protein VP2, capsid protein VP3, capsid protein VP4, protease 2A, protease 3C, protein 2B, protein 2C, protein 3A, protein 3AB, protein 3CD, viral protein genome-linked

Camel

Virus

-20°C,2°C to 8°C

12352203