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ProductsBack/Anti-GH protein [9D5D2A9]

Anti-GH protein [9D5D2A9]

AB03396
Absolute Antibody
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetGH1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-GH protein [9D5D2A9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody 9D5D2A9 and the commercial rabbit polyclonal antibody were used to stain pituitary tumor. Results showed the antibody specificity is comparable to the commercial antibody. Further, the sensitivity of the antibody 9D5D2A9 was higher than the commercial antibody. The antibody and the commercially available rabbit polyclonal antibody were simultaneously detected on the normal tissue chip, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody (CN113845595A).
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    9D5D2A9
  • Gene ID2688
  • Target name
    GH1
  • Target description
    growth hormone 1
  • Target synonyms
    GH; GHB5; GHN; GH-N; growth hormone B5; hGH-N; IGHD1A; IGHD1B; IGHD2; pituitary growth hormone; somatotropin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01241
  • Protein Name
    Somatotropin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GH1 using anti-GH1 antibody (A00851-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat C6 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: mouse RAW264.7 whole cell lysates, Lane 7: mouse 3T3-L1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GH1 antigen affinity purified polyclonal antibody (Catalog # A00851-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 22 kDa. The expected band size for GH1 is at 25 kDa.
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