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Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.

Anti-GIGYF1 Antibody

HPA020999
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetGIGYF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GIGYF1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64599
  • Target name
    GIGYF1
  • Target description
    GRB10 interacting GYF protein 1
  • Target synonyms
    GRB10-interacting GYF protein 1; GYF1; PERQ amino acid-rich with GYF domain-containing protein 1; PERQ1; postmeiotic segregation increased 2-like 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75420
  • Protein Name
    GRB10-interacting GYF protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GIGYF1 using anti-GIGYF1 antibody (A14236). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIGYF1 antigen affinity purified polyclonal antibody (Catalog # A14236) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIGYF1 at approximately 150 kDa. The expected band size for GIGYF1 is at 115 kDa.
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