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Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum.

Anti-GIMAP1 Antibody

HPA053441
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetGIMAP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GIMAP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID170575
  • Target name
    GIMAP1
  • Target description
    GTPase, IMAP family member 1
  • Target synonyms
    HIMAP1, IAN2, IMAP1, IMAP38, GTPase IMAP family member 1, immune-associated nucleotide-binding protein 2, immunity associated protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WWP7
  • Protein Name
    GTPase IMAP family member 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of GIMAP1 using anti-GIMAP1 antibody (A11144-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GIMAP1 antigen affinity purified polyclonal antibody (Catalog # A11144-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GIMAP1 at approximately 36 kDa. The expected band size for GIMAP1 is at 34 kDa.
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