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Immunohistochemical staining of human lymph group shows moderate nuclear positivity in germinal center cells.
Immunohistochemical staining of human lymph group shows moderate nuclear positivity in germinal center cells.
Immunohistochemical staining of human lymph group shows moderate nuclear positivity in germinal center cells.

Anti-GINS2 Antibody

HPA057285
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGINS2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GINS2 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51659
  • Target name
    GINS2
  • Target description
    GINS complex subunit 2
  • Target synonyms
    HSPC037, PSF2, Pfs2, DNA replication complex GINS protein PSF2, GINS complex subunit 2 (Psf2 homolog)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y248
  • Protein Name
    DNA replication complex GINS protein PSF2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PSF2/GINS2 using anti-PSF2/GINS2 antibody (A09943-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSF2/GINS2 antigen affinity purified polyclonal antibody (Catalog # A09943-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSF2/GINS2 at approximately 21 kDa. The expected band size for PSF2/GINS2 is at 21 kDa.
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