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Immunohistochemical staining of human heart muscle shows moderate membranous and cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate membranous and cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate membranous and cytoplasmic positivity in myocytes.

Anti-GJA4 Antibody

HPA047981
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGJA4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GJA4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2701
  • Target name
    GJA4
  • Target description
    gap junction protein alpha 4
  • Target synonyms
    CX37, gap junction alpha-4 protein, connexin-37, gap junction protein, alpha 4, 37kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35212
  • Protein Name
    Gap junction alpha-4 protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GJA4 using anti-GJA4 antibody (A05569-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GJA4 antigen affinity purified polyclonal antibody (Catalog # A05569-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GJA4 at approximately 37 kDa. The expected band size for GJA4 is at 37 kDa.
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