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Immunofluorescence staining of mouse periaqueductal grey shows immunoreactivity in neuronal cell bodies.
Immunofluorescence staining of mouse periaqueductal grey shows immunoreactivity in neuronal cell bodies.
Immunofluorescence staining of mouse periaqueductal grey shows immunoreactivity in neuronal cell bodies.

Anti-GKAP1 Antibody

HPA035117
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetGKAP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GKAP1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80318
  • Target name
    GKAP1
  • Target description
    G kinase anchoring protein 1
  • Target synonyms
    cGMP-dependent protein kinase anchoring protein 42kDa; cGMP-dependent protein kinase-anchoring protein of 42 kDa; FKSG21; G kinase-anchoring protein 1; GKAP42; protein kinase anchoring protein GKAP42
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5VSY0
  • Protein Name
    G kinase-anchoring protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GKAP1 using anti-GKAP1 antibody (A14397-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GKAP1 antigen affinity purified polyclonal antibody (Catalog # A14397-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GKAP1 at approximately 40-42 kDa. The expected band size for GKAP1 is at 42 kDa.
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