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Immunohistochemical staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.

Anti-GLG1 Antibody

HPA010815
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetGLG1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GLG1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2734
  • Target name
    GLG1
  • Target description
    golgi glycoprotein 1
  • Target synonyms
    CFR-1; cysteine-rich fibroblast growth factor receptor; E-selectin ligand 1; ESL-1; Golgi apparatus protein 1; golgi sialoglycoprotein MG-160; MG160; MG-160
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92896
  • Protein Name
    Golgi apparatus protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GLG1 using anti-GLG1 antibody (A07510-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLG1 antigen affinity purified polyclonal antibody (Catalog # A07510-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLG1 at approximately 150 kDa. The expected band size for GLG1 is at 135 kDa.
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