Anti-Gliadin [VHH26]

Absolute Antibody

Anti-Gliadin [VHH26]

9

This antibody specifically recognizes and binds and epitope unique to wheat alpha gliadins.

This antibody specifically binds an epitope unique for wheat alpha gliadins which is not present in gamma or omega gliadins. It does not cross reat with rye or barley gliadins. VHH26 can detect gliadins in denaturing conditions and it retains its binding capacity in presence of 15% ethanol, 0.5% 2-mercaptoethanol, and 0.5 M guanidinium chloride. Capture ELISA using VHH26 was able to detect gliadins in samples shown as negatives by conventional ELISA. This antibody can be used for detection of gluten in commercial food samples using ELISA (limit of detection 0.24 ppm) and by competitive ELISA using conventional antibodies (limit of detection: 1 ppm) (PMID: 20039674).

ELISA

Research Use Only

Monoclonal

VHH26

Mouse

IgG1

This chimeric mouse antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Plant

-20°C,2°C to 8°C

12352203