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Immunohistochemical staining of human thyroid gland shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate nuclear positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate nuclear positivity in glandular cells.

Anti-GLIS3 Antibody

HPA056426
Atlas Antibodies
ApplicationsWestern Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGLIS3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GLIS3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID169792
  • Target name
    GLIS3
  • Target description
    GLIS family zinc finger 3
  • Target synonyms
    NDH, ZNF515, zinc finger protein GLIS3, GLI-similar 3, OK/KNS-cl.4, zinc finger protein 515
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NEA6
  • Protein Name
    Zinc finger protein GLIS3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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IHC image of CSB-PA839863LA01HU diluted at 1:300 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of GLIS3 using anti-GLIS3 antibody (A03710-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLIS3 antigen affinity purified polyclonal antibody (Catalog # A03710-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLIS3 at approximately 84 kDa. The expected band size for GLIS3 is at 84 kDa.
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