Anti-Glycoprotein E2 [HC33.1]

Absolute Antibody

Anti-Glycoprotein E2 [HC33.1]

7

This broadly neutralizing antibody successfully neutralized JFH1 2a HCVcc and HCVcc isolates from genotypes 1a, 4a, and 5a. This antibody was found inhibit the binding of E2 glycoprotein to CD81 in a dose dependent manner. The binding affinity of the scFv version of this antibody was determined by using surface plasmon resonance in a BIAcore 3000. The binding specificity of the antibody to native or denatured recombinant E2 was determined by ELISA. Epitope mapping of the antibody was also done using ELISA (PMID: 23097455). HC33.1 was also reported to neutralize virus bearing the E2 N417S and N417T adaptive mutations with the glycosylation shift to Asn-415 (PMID: 25737449). This antibody was used in a western blot analysis to identify HEK293 and Sf9 derived sE2 (PMID: 30651366).

Western Blot, ELISA, Neutralisation/Blocking

ELISA; neutralize; WB

Research Use Only

Genome polyprotein

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

-20°C,2°C to 8°C

12352203