Anti-GM1-bound Abeta [4396], Mouse IgM, lambda

Absolute Antibody

Anti-GM1-bound Abeta [4396], Mouse IgM, lambda

9

This antibody is specific for human GM1-Abeta.

The original mouse IgM antibody was developed and tested to specifically recognize Abeta bound to GM1 (GM1-Abeta). A subsequent version, 4396C, an IgG2a subtype chimeric antibody, was generated and evaluated. The chimeric antibody retained its specificity for GM1-Abeta, as demonstrated by electron microscopy (EM), where it specifically labeled GM1-containing liposomes incubated with Abeta; Western blotting (WB), which confirmed the antibodys specificity for GM1-Abeta; a Thioflavin T (ThT) assay, which showed that the antibody inhibited amyloid fibril formation in a dose-dependent manner when incubated with GM1-containing liposomes and Abeta; and immunoelectron microscopy (IM), where it effectively bound to newly formed amyloid fibrils in the presence of synthetic Abeta and GM1-containing liposomes (US7339035B2). The chimeric antibody was assessed in three polymerization systems: (1) polymerization initiated by adding Abeta fibril fragment, (2) Abeta40 polymerization initiated from GAbeta40, and (3) Abeta42 polymerization initiated from GAbeta42. It exhibited suppression of the Abeta extension (US7741448B2).

Electron Microscopy, ImmunoFluorescence, Western Blot, Other Application

Research Use Only

Monoclonal

4396

Mouse

IgM

Human

-20°C,2°C to 8°C

12352203