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Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern.
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in granular pattern.

Anti-GNS Antibody

HPA013695
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGNS
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GNS
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2799
  • Target name
    GNS
  • Target description
    glucosamine (N-acetyl)-6-sulfatase
  • Target synonyms
    G6S, N-acetylglucosamine-6-sulfatase, glucosamine -6-sulfatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15586
  • Protein Name
    N-acetylglucosamine-6-sulfatase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GNS using anti-GNS antibody (A00999-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNS antigen affinity purified polyclonal antibody (Catalog # A00999-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GNS at approximately 90KD. The expected band size for GNS is at 62KD.
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