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ProductsBack/Anti-GPC3 [GC199]

Anti-GPC3 [GC199]

Ab01210-1.1
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetGPC3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-GPC3 [GC199]
  • Delivery Days Customer
    7
  • Application Supplier Note
    ELISA was used to confirm the specificity of GC199 for GPC3.
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GC199
  • Gene ID2719
  • Target name
    GPC3
  • Target description
    glypican 3
  • Target synonyms
    DGSX; glypican proteoglycan 3; glypican-3; GTR2-2; heparan sulphate proteoglycan; intestinal protein OCI-5; MXR7; OCI-5; SDYS; secreted glypican-3; SGB; SGBS; SGBS1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP51654
  • Protein Name
    Glypican-3
  • Scientific Description
    This reformatted mouse antibody was made using the variable domain sequences of the original Mouse format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Balb/c mice were immunised with GC-3, a GST-fusion protein.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Glypican 3/GPC3 using anti-Glypican 3/GPC3 antibody (A01922-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 3/GPC3 antigen affinity purified polyclonal antibody (Catalog # A01922-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glypican 3/GPC3 at approximately 66 kDa. The expected band size for Glypican 3/GPC3 is at 66 kDa.
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