Figure 1. Western blot analysis of GPCR LGR8 using anti-GPCR LGR8 antibody (A04848-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SHG-44 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR LGR8 antigen affinity purified polyclonal antibody (Catalog # A04848-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR LGR8 at approximately 86KD. The expected band size for GPCR LGR8 is at 86KD.
Figure 1. Western blot analysis of GPCR LGR8 using anti-GPCR LGR8 antibody (A04848-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SHG-44 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPCR LGR8 antigen affinity purified polyclonal antibody (Catalog # A04848-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPCR LGR8 at approximately 86KD. The expected band size for GPCR LGR8 is at 86KD.
Anti-GPCR LGR8/RXFP2 Antibody Picoband(r)
A04848-1-BIOTIN
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetRXFP2
Overview
- SupplierBoster Bio
- Product NameAnti-GPCR LGR8/RXFP2 Antibody Picoband(r)
- Delivery Days Customer9
- Antibody SpecificityNo cross reactivity with other proteins.
- ApplicationsFlow Cytometry, Western Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- ConjugateBiotin
- Gene ID122042
- Target nameRXFP2
- Target descriptionrelaxin family peptide receptor 2
- Target synonymsG protein coupled receptor affecting testicular descent; GPR106; G-protein coupled receptor 106; GREAT; INSL3R; leucine-rich repeat-containing G-protein coupled receptor 8; LGR8; LGR8.1; relaxin receptor 2; relaxin/insulin like family peptide receptor 2; RXFPR2
- HostRabbit
- IsotypeIgG
- Protein IDQ8WXD0
- Protein NameRelaxin receptor 2
- Scientific DescriptionBoster Bio Anti-GPCR LGR8/RXFP2 Antibody Picoband® catalog # A04848-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203