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Anti-GPX4 Antibody

ER1803-15
HUABIO
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
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Product group Antibodies
Price on request
100 ul
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Overview

  • Supplier
    HUABIO
  • Product Name
    Anti-GPX4 Antibody
  • Delivery Days Customer
    7
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB,IF-Cell,IHC-P,FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/mL.
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
  • UNSPSC
    12352203

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Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Heart tissue lysates ; Lane 5: Rat Testis tissue lysates ; Lane 6: Rat Liver tissue lysates; Lane 7: Rat Kidney tissue lysates; Lane 8: Rat Heart tissue lysates; Lane 9: U937 cell lysates; Lane 10: HepG2 cell lysates; Lane 11: MCF-7 cell lysates; Lane 12: Huvec cell lysates; Lane 13: 293T cell lysates probed with GPX4 Polyclonal Antibody, Unconjugated (bs-3884R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (A02059-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody (Catalog # A02059-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 19 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 19 kDa.
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