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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA03049A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA03049A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA03049A0Rb at dilution of 1:100

GPX4 Antibody

CSB-PA03049A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGPX4
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Overview

  • Supplier
    Cusabio
  • Product Name
    GPX4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2879
  • Target name
    GPX4
  • Target description
    glutathione peroxidase 4
  • Target synonyms
    epididymis secretory sperm binding protein; GPx-4; GSHPx-4; MCSP; PHGPx; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; selenoprotein GPX4; SMDS; snGPx; snPHGPx; sperm nucleus glutathione peroxidase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP36969
  • Protein Name
    Phospholipid hydroperoxide glutathione peroxidase
  • Scientific Description
    Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Essential for maturation and survival of photoreceptor cells. Plays a role in a primary T cell response to viral and parasitic infection by protecting T cells from ferroptosis, a cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species, and by supporting T cell expansion.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Heart tissue lysates ; Lane 5: Rat Testis tissue lysates ; Lane 6: Rat Liver tissue lysates; Lane 7: Rat Kidney tissue lysates; Lane 8: Rat Heart tissue lysates; Lane 9: U937 cell lysates; Lane 10: HepG2 cell lysates; Lane 11: MCF-7 cell lysates; Lane 12: Huvec cell lysates; Lane 13: 293T cell lysates probed with GPX4 Polyclonal Antibody, Unconjugated (bs-3884R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Glutathione Peroxidase 4/GPX4 using anti-Glutathione Peroxidase 4/GPX4 antibody (A02059-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: monkey COS-7 whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glutathione Peroxidase 4/GPX4 antigen affinity purified polyclonal antibody (Catalog # A02059-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glutathione Peroxidase 4/GPX4 at approximately 19 kDa. The expected band size for Glutathione Peroxidase 4/GPX4 is at 19 kDa.
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