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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.

Anti-GRIPAP1 Antibody

HPA000615
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGRIPAP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-GRIPAP1
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID56850
  • Target name
    GRIPAP1
  • Target description
    GRIP1 associated protein 1
  • Target synonyms
    GRASP-1, GRIP1-associated protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ4V328
  • Protein Name
    GRIP1-associated protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRASP1/GRIPAP1 using anti-GRASP1/GRIPAP1 antibody (A13005-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: huamn HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat panceras tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse pancreas tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRASP1/GRIPAP1 antigen affinity purified polyclonal antibody (Catalog # A13005-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRASP1/GRIPAP1 at approximately 96 kDa. The expected band size for GRASP1/GRIPAP1 is at 96 kDa.
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