Anti-GXM [18B7]

Absolute Antibody

Anti-GXM [18B7]

7

To characterize the mouse version of this antibody an ELISA was performed on native and de-O-acetylated serotype A GMX using the mouse version of this antibody (Casadevall et al, 1992; pmid:1583327). Further immunofluorescence was performed on yeast cells using the mouse version of this antibody (Casadevell et al, 1998; pmid:9624491). Binding of the mouse version of this antibody to normal and C. neoformans-infected tissues was studied by immunohistochemistry (Casadevell et al, 1998; pmid:9624491). The murine macrophage-like cell line J774.16 was used to determine the ability of the mouse version of this antibody to opsonize C. neoformans (Casadevell et al, 1998; pmid:9624491). The effect of the murine version of this antibody on human PMN phagocytosis of C. neoformans was measured by a flow cytometry (Casadevell et al, 1998; pmid:9624491). C3 binding studies were done with formalin-killed cells of strain 388. The kinetics were studied both in the presence and absence of the murine version of this antibody (Casadevell et al, 1998; pmid:9624491). The ability of the mouse version of this antibody to reduce serum antigen in A/J mice was tested (Casadevell et al, 1998; pmid:9624491). The mouse version of this antibody has shown promise in a phase 1 trial testing patients with treated cryptococcal meningitis (Larsen et al, 2005; pmid:15728888). While developing a diagnostic test for Cryptococcal capsular antigens. The mouse version of this antibody was used for ELISA to test cross-reactivity with capsid antigens of other yeast species (Sathongdejwisit et al, 2021; pmid:33922698).

Functional Assay, Flow Cytometry, ELISA, ImmunoHistoChemistry

FC; ELISA; IHC; functional assay; therapeutic

Research Use Only

Monoclonal

18B7

Mouse

IgG1

Fungus

Cryptococcus neoformans

The original mouse version of this antibody was raised by infecting balb/c mice with C. neoformans and then immunizing them with glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT).

-20°C,2°C to 8°C

41116161