Anti-GXM [18B7]

Absolute Antibody

Anti-GXM [18B7]

7

To characterize the mouse version of this antibody an ELISA was performed on native and de-O-acetylated serotype A GMX using the mouse version of this antibody (Casadevall et al, 1992; pmid:1583327). Further immunofluorescence was performed on yeast cells using the mouse version of this antibody (Casadevell et al, 1998; pmid:9624491). Binding of the mouse version of this antibody to normal and C. neoformans-infected tissues was studied by immunohistochemistry (Casadevell et al, 1998; pmid:9624491). The murine macrophage-like cell line J774.16 was used to determine the ability of the mouse version of this antibody to opsonize C. neoformans (Casadevell et al, 1998; pmid:9624491). The effect of the murine version of this antibody on human PMN phagocytosis of C. neoformans was measured by a flow cytometry (Casadevell et al, 1998; pmid:9624491). C3 binding studies were done with formalin-killed cells of strain 388. The kinetics were studied both in the presence and absence of the murine version of this antibody (Casadevell et al, 1998; pmid:9624491). The ability of the mouse version of this antibody to reduce serum antigen in A/J mice was tested (Casadevell et al, 1998; pmid:9624491). The mouse version of this antibody has shown promise in a phase 1 trial testing patients with treated cryptococcal meningitis (Larsen et al, 2005; pmid:15728888). While developing a diagnostic test for Cryptococcal capsular antigens. The mouse version of this antibody was used for ELISA to test cross-reactivity with capsid antigens of other yeast species (Sathongdejwisit et al, 2021; pmid:33922698).

Functional Assay, Flow Cytometry, ELISA, ImmunoHistoChemistry

FC; ELISA; IHC; functional assay; therapeutic

Research Use Only

Monoclonal

18B7

Rabbit

IgG

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Fungus

Cryptococcus neoformans

The original mouse version of this antibody was raised by infecting balb/c mice with C. neoformans and then immunizing them with glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT).

-20°C,2°C to 8°C

41116161