Anti-H-2Kb MHC [AF6-88.5.5.3]

Absolute Antibody

Anti-H-2Kb MHC [AF6-88.5.5.3]

7

This antibody is specific for mouse H-2 class I histocompatibility antigen. H-2 class I histocompatibility antigen is involved in the presentation of foreign antigens to the immune system.

Cloned mouse Th cells L2, grown on CBA/J stimulating spleen cells were used for flow cytometry using this antibody (Wall et al, 1983; pmid:6193171). The inhibition of 125I-7 1-54.3 binding to mouse spleen cel was tested using this antibody (Wall et al, 1983; pmid:6193171). Cells isolated from the spleen, BM, and peripheral blood of transplanted and control animals were assayed by flow cytometry using this antibody (Bjornson et al, 1999; pmid:9915700). Mouse spleen leukocytes were analyzed in flow cytometry using this antibody (Xie et al, 2006; pmid:17050600). Plenic lymphocytes derived from transgenic mice expressing wild-type (WT) H-2Kb, deltaY or delta7. H-2Kb were used for immunoprecipitation. In this procedure the proteins were immunoprecipitated using this antibody (Lizee et al, 2003; pmid:14566337). Peripheral blood leukocytes from mice were used in a flow cytometry assay using this antibody (Lizee et al, 2003; pmid:14566337). Immunofluorescence was preformed on mouse mature spleen-derived DCs using this antibody (Lizee et al, 2003; pmid:14566337). M1 cells, a clonal isolate derived from B16-F10 murine melanoma cells, were immunostained using this antibody (Hauser et al, 1993; pmid:8467514).

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Neutralisation/Blocking

FC; inhibition; IF; IP

Research Use Only

Monoclonal

AF6-88.5.5.3

H2-K1

histocompatibility 2, K1, K region

class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H2-D1; H-2K; H2-K; H-2K(B); H-2K(d); H-2K(K); H-2K(Q); H2-K_p; histocompatibility 2, K region, promoter 1; K-f; MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC class I heavy chain heavy chain; MHC class I heavy chain maturation peptide; MHC class II antigen D; MHC class II antigen K; MHC H2-K transplantation antigen

Rabbit

IgG

H-2 class I histocompatibility antigen, K-B alpha chain

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Mouse

Mouse

This antibody was produced by the fusion of mouse myeloma Sp2/0 cells with spleen cells from a rat, that was immunized with the H-2 class I histocompatibility antigen.

-20°C,2°C to 8°C

12352203