Anti-HA [5J8]

Absolute Antibody

Anti-HA [5J8]

7

This antibody is specific for the H1N1 influenza virus hemagglutinin protein. It recognizses a conserved non-linear epitope (residues 133, 137 and 222) on the globular head of H1N1 HA between the receptor-binding pocket and the Ca2 antigenic site. The ant

The crystal structure of the Fab fragment in complex with a bacterially expressed and refolded globular head domain from the hemagglutinin was determined (Hong et al., 2013; PMID: 24027321). The antibody was inhibitory in a hemagglutination inhibition activity against H1N1pdm2009-like virus. Neutralization capacity of the antibody was prooved by an MN-assay. The specificity of the antibody to the monomeric head as to the full HA-trimer was confirmed by ELISA (Aartse et al., 2021; PMID: 34358138).

ELISA, Neutralisation/Blocking, Other Application

in vivo; ELISA; Neutralization; Inhibition; Crystallization

Research Use Only

Monoclonal

5J8

Human

IgG1

Virus

H1N1 Influenza A virus

The original antibody was isolated from the peripheral blood mononuclear cells of a healthy 47-year-old human subject.

-20°C,2°C to 8°C

12352203