Bio-Connect

Anti-Haemoglobin [2A1]

Research Use Only
Ab00956-23.0
Absolute Antibody
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Haemoglobin [2A1]
  • Delivery Days Customer
    7
  • Antibody Specificity
    2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin
  • Application Supplier Note
    2A1 has been used to bind to mHb in Western Blots and has been found to increase the secretion of TNF-alpha from endothelial cells when co-incubated with Hb, suggesting that 2A1 induces pro-inflammatory events (Bhatnagar 2011).
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01942
  • Protein Name
    Hemoglobin subunit alpha
  • Scientific Description
    This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Reactivity Supplier Note
    Murine hybridomas were generated using spleen cells from aging (8–10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend’s Adjuvant.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203