Anti-Haemoglobin [2A1]
Ab00956-23.0
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse
Overview
- SupplierAbsolute Antibody
- Product NameAnti-Haemoglobin [2A1]
- Delivery Days Customer7
- Antibody Specificity2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 frmo the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin
- Application Supplier Note2A1 has been used to bind to mHb in Western Blots and has been found to increase the secretion of TNF-alpha from endothelial cells when co-incubated with Hb, suggesting that 2A1 induces pro-inflammatory events (Bhatnagar 2011).
- ApplicationsWestern Blot, ELISA
- Applications SupplierELISA; WB
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A1
- HostRabbit
- IsotypeIgG
- Protein IDP01942
- Protein NameHemoglobin subunit alpha
- Scientific DescriptionThis chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
- ReactivityMouse
- Reactivity SupplierMouse
- Reactivity Supplier NoteMurine hybridomas were generated using spleen cells from aging (8–10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend’s Adjuvant.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203