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Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in neuronal cells.

Anti-HAUS3 Antibody

HPA048190
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetHAUS3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HAUS3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79441
  • Target name
    HAUS3
  • Target description
    HAUS augmin like complex subunit 3
  • Target synonyms
    C4orf15; dgt3; HAUS augmin-like complex subunit 3; IT1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ68CZ6
  • Protein Name
    HAUS augmin-like complex subunit 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HAUS3 using anti-HAUS3 antibody (A12796-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAUS3 antigen affinity purified polyclonal antibody (Catalog # A12796-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAUS3 at approximately 55 kDa. The expected band size for HAUS3 is at 70 kDa.
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