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Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of white pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of white pulp.
Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells of white pulp.

Anti-HCLS1 Antibody

HPA019143
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHCLS1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HCLS1 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3059
  • Target name
    HCLS1
  • Target description
    hematopoietic cell-specific Lyn substrate 1
  • Target synonyms
    CTTNL, HS1, lckBP1, p75, hematopoietic lineage cell-specific protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14317
  • Protein Name
    Hematopoietic lineage cell-specific protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HCLS1 using anti-HCLS1 antibody (A04313-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCLS1 antigen affinity purified polyclonal antibody (Catalog # A04313-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCLS1 at approximately 75 kDa. The expected band size for HCLS1 is at 54 kDa.
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