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Figure 2. IHC analysis of HCN2 using anti-HCN2 antibody (A02804). HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-HCN2 Antibody (A02804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of HCN2 using anti-HCN2 antibody (A02804). HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-HCN2 Antibody (A02804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of HCN2 using anti-HCN2 antibody (A02804). HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-HCN2 Antibody (A02804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Anti-HCN2 Antibody Picoband(r)

A02804
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetHCN2
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-HCN2 Antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB, IHP, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID610
  • Target name
    HCN2
  • Target description
    hyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2
  • Target synonyms
    BCNG2; BCNG-2; brain cyclic nucleotide-gated channel 2; EIG17; FEB2; GEFSP11; HAC-1; hyperpolarization activated cyclic nucleotide gated potassium channel 2; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UL51
  • Protein Name
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
  • Scientific Description
    Boster Bio Anti-HCN2 Antibody Picoband® catalog # A02804. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity Supplier
    Mouse, Rat,Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The HCN domain is required for HCN channel cell-surface expression and couples voltage- and cAMP-dependent gating mechanisms.
    Read more
  • Spironolactone diminishes spontaneous ventricular premature beats by reducing HCN4 protein expression in rats with myocardial infarction.
    Read more
  • Dynamic changes in HCN2, HCN4, KCNE1, and KCNE2 expression in ventricular cells from acute myocardial infarction rat hearts.
    Read more

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Figure 2. IHC analysis of HCN2 using anti-HCN2 antibody (A02804). HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-HCN2 Antibody (A02804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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