Figure 2. IHC analysis of HCN2 using anti-HCN2 antibody (A02804). HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-HCN2 Antibody (A02804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of HCN2 using anti-HCN2 antibody (A02804). HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-HCN2 Antibody (A02804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-HCN2 Antibody
A02804
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHCN2
Overview
- SupplierBoster Bio
- Product NameAnti-HCN2 Antibody
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- Applications SupplierWB, IHP, IHC
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID610
- Target nameHCN2
- Target descriptionhyperpolarization activated cyclic nucleotide gated potassium and sodium channel 2
- Target synonymsBCNG-2, BCNG2, EIG17, FEB2, GEFSP11, HAC-1, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2, brain cyclic nucleotide-gated channel 2, hyperpolarization activated cyclic nucleotide gated potassium channel 2
- HostRabbit
- IsotypeIgG
- Protein IDQ9UL51
- Protein NamePotassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2
- Scientific DescriptionBoster Bio Anti-HCN2 Antibody Picoband® catalog # A02804. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Mouse, Rat
- Reactivity SupplierMouse, Rat,Human
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Wang ZJ, Blanco I, Hayoz S, et al. The HCN domain is required for HCN channel cell-surface expression and couples voltage- and cAMP-dependent gating mechanisms. J Biol Chem. 2020,295(24):8164-8173. doi: 10.1074/jbc.RA120.013281Read this paper
- Song T, Yang J, Yao Y, et al. Spironolactone diminishes spontaneous ventricular premature beats by reducing HCN4 protein expression in rats with myocardial infarction. Mol Med Rep. 2011,4(3):569-73. doi: 10.3892/mmr.2011.455Read this paper
- Xia S, Wang Y, Zhang Y, et al. Dynamic changes in HCN2, HCN4, KCNE1, and KCNE2 expression in ventricular cells from acute myocardial infarction rat hearts. Biochem Biophys Res Commun. 2010,395(3):330-5. doi: 10.1016/j.bbrc.2010.04.003Read this paper
Datasheet
MSDS
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