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Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells and projections.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells and projections.
Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in neuronal cells and projections.

Anti-HCN4 Antibody

HPA073174
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHCN4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HCN4 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10021
  • Target name
    HCN4
  • Target description
    hyperpolarization activated cyclic nucleotide gated potassium channel 4
  • Target synonyms
    BRGDA8, EIG18, SSS2, potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, hyperpolarization activated cyclic nucleotide-gated cation channel 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y3Q4
  • Protein Name
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HCN4 using anti-HCN4 antibody (A02235-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCN4 antigen affinity purified polyclonal antibody (Catalog # A02235-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCN4 at approximately 140 kDa. The expected band size for HCN4 is at 129 kDa.
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