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Immunohistochemical staining of human hypothalamus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hypothalamus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human hypothalamus shows strong cytoplasmic positivity in neuronal cells.

Anti-HCRT Antibody

HPA064801
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetHCRT
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HCRT
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3060
  • Target name
    HCRT
  • Target description
    hypocretin neuropeptide precursor
  • Target synonyms
    hypocretin (orexin) neuropeptide; NRCLP1; orexin; OX; PPOX; prepro-orexin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43612
  • Protein Name
    Orexin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. IHC analysis of HCRT using anti-HCRT antibody (A01633-1). HCRT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 microg/ml rabbit anti-HCRT Antibody (A01633-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA090698(HCRT Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
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