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Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Anti-HDDC2 Antibody

HPA035677
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetHDDC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HDDC2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51020
  • Target name
    HDDC2
  • Target description
    HD domain containing 2
  • Target synonyms
    5'-deoxynucleotidase HDDC2; C6orf74; CGI-130; dJ167O5.2; HCV NS5A-transactivated protein 2; HD domain-containing protein 2; hepatitis C virus NS5A-transactivated protein 2; NS5ATP2; testicular tissue protein Li 83
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z4H3
  • Protein Name
    HD domain-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDDC2 using anti-HDDC2 antibody (A08033-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDDC2 antigen affinity purified polyclonal antibody (Catalog # A08033-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDDC2 at approximately 26 kDa. The expected band size for HDDC2 is at 23 kDa.
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