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Anti-HDGF [H3]

Ab02457-1.1
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetHDGF
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-HDGF [H3]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody was used for immunoprecipitation on total cell lysates from H460 NSCLC cells (US20070243191). The neutralizing ability of this antibody was tested on A549. The result was a significant decrease in tumor size (US20070243191). This antibody was fluorescently labelled and injected into nude mice to test the antibody distribution. The antibodies evenly distributed in the animals including the tumors. This antibody entered into the cytoplasmic compartment of some tumor cells (US20070243191). Immunohistochemistry with this antibody was performed on tissues from NSCLC patients (US20070243191). Western blot was performed on HDGF protein using this antibody (Ren et al, 2009;pmid:19435872). ELISA was performed on the HDGF protein using this antibody (US20070243191).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, Neutralisation/Blocking
  • Applications Supplier
    IP; IF; IHC; neutralizing; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    H3
  • Gene ID3068
  • Target name
    HDGF
  • Target description
    heparin binding growth factor
  • Target synonyms
    epididymis secretory sperm binding protein; hepatoma derived growth factor; hepatoma-derived growth factor; high mobility group protein 1-like 2; HMG1L2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP51858
  • Protein Name
    Hepatoma-derived growth factor
  • Reactivity Supplier
    Human, mouse
  • Reactivity Supplier Note
    The original antibody was raised by immunizing mice with full-length recombinant HDGF. This antibody binds a different epitope on HDGF than antibody C1.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDGF using anti-HDGF antibody (A01057). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDGF antigen affinity purified polyclonal antibody (Catalog # A01057) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDGF at approximately 38 kDa. The expected band size for HDGF is at 27 kDa.
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