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Anti-Heart- type fatty acid-binding protein [HF10]

AB03724-10.0
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetFABP3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Heart- type fatty acid-binding protein [HF10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The reactivity of this antibody towards human H-FABP was tested using ELISA. This antibody can be used in combination with another antibody 11F2 for detection of H-FABP in a sample using sandwich ELISA. HF2 is used as coating antibody, and HF10 is labeled with HRP for detection. This antibody combination was also used for the preparation of a myocardial infarction kit for detection of H-FAPB in the sera of 26 patients (CN1978465).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HF10
  • Gene ID2170
  • Target name
    FABP3
  • Target description
    fatty acid binding protein 3
  • Target synonyms
    epididymis secretory sperm binding protein; FABP11; fatty acid binding protein 11; fatty acid binding protein 3, muscle and heart; fatty acid-binding protein, heart; heart-type fatty acid-binding protein; H-FABP; mammary-derived growth inhibitor; MDGI; M-FABP; muscle fatty acid-binding protein; O-FABP
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP05413
  • Protein Name
    Fatty acid-binding protein, heart
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody (A01734-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody (Catalog # A01734-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15 kDa. The expected band size for Cardiac FABP is at 15 kDa.
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