Anti-Hemagglutinin 5 [H5.3]

Absolute Antibody

Anti-Hemagglutinin 5 [H5.3]

9

H5.3 binds to H5 at the head domain at an epitope which involves the 190 loop. The epitope was determined to be LGVSSA (aa 134-139) and YQGKSS (aa 142-147) of the head domain by DXMS (deuterium exchange mass spectrometry). This antibody shows reactivity w

H5.3 binds to H5 where it is able to block the receptor binding site on HA by inserting its CDR3 into the site (IC50 ~ 20 ng/ml). H5.3 can neutralize wild-type VN/1203, rdt VN/1203 K and VN/1203 F pseudovirions, all with IC50s of 5 ng/ml. This antibody also has potent HI (hemagglutination inhibition) activity (80 ng/ml).

Neutralisation/Blocking

Research Use Only

Monoclonal

H5.3

Human

IgG1

Hemagglutinin

This reformatted human antibody was made using the variable domain sequences of the original Human IgG format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203