Anti-Hemin [1D3]

Absolute Antibody

Anti-Hemin [1D3]

9

The antibody is specific to metal-substituted protoporphyrin. CDRH2 plays an essential role in recognition. The antibody has the highest affinity for PP[Fe]-PEG and a five-times lower affinity for PP[Pd]-PEG, PP[Zn]-PEG, and PP[Ga]-PEG.

The affinity of this antibody for hemin has been characterized by ELISA (Uda et al., 1993). The affinity constant of the antibody and individual CDR segments has been examined with fluorescence measurement of protoporphyrin-PEG binding (Hifumi et al., 1999). The mouse IgM version of this antibody (Ab00982-21.0) was used as a primary antibody for immunocytochemistry (IHC) in myelinating cultures after treatment with hemin/FeCl3 (Baldacchino et al., 2022; PMID: 35569511).

ImmunoFluorescence, ELISA, ImmunoHistoChemistry

Research Use Only

Monoclonal

1D3

Human

IgG1

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgM format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203