Anti-Hendra and Nipah virus F [1F5]

Absolute Antibody

Anti-Hendra and Nipah virus F [1F5]

9

This antibody binds a pre-fusion conformational epitope on the F glycoprotein of the Nipah and Hendra virus. The Nipah and Hendra virus are spread by bats.

This antibody is reported to broadly neutralize Nipah and Hendra virus by blocking membrane fusion. The cryogenic-electron microscopy (cryo-EM) structures of the antibody in complex with HeV F was determined. Biolayer interferometry was used to characterize the binding of the 1F5 Fab fragments to prefusion NiV F or HeV F ectodomain trimers immobilized on the surface of biosensors. The equilibrium dissociation constants (Kd) of 1.47 and 1.24 nM for attachment of Fab fragments of 1F5 to NiV F or HeV F, respectively. To further characterize this antibody, a kinetic assay was performed on HeV F- or NiV F- or NiV F S69A using the mouse version of this antibody. To analyze the ability of this antibody to prevent and cure disease, a fusion inhibition assay was performed on CHO745 cells transfected with HNV F and G glycoproteins using the mouse IgG version of this antibody. Further a plaque reduction assay was performed NiV-M, NiV-B or HeV using both the human and mouse IgG versions of this antibody. The humanized version of the antibody broadly inhibited NiV-M, NiV-B and HeV with IC50 ranging between 0.2 and 1.3 microg/ml (Dang et al., 2021; PMID:33927387).

Functional Assay, Neutralisation/Blocking

Research Use Only

Monoclonal

1F5

Mouse

IgG1

Virus

-20°C,2°C to 8°C

12352203