Figure 2. IHC analysis of Hepatitis B Virus using anti-Hepatitis B Virus antibody (A30379). Hepatitis B Virus was detected in paraffin-embedded section of human hepatitis B tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Hepatitis B Virus Antibody (A30379) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 2. IHC analysis of Hepatitis B Virus using anti-Hepatitis B Virus antibody (A30379). Hepatitis B Virus was detected in paraffin-embedded section of human hepatitis B tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-Hepatitis B Virus Antibody (A30379) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Anti-Hepatitis B Virus Antibody Picoband(r)
A30379
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Virus
TargetS
Overview
- SupplierBoster Bio
- Product NameAnti-Hepatitis B Virus Antibody Picoband(r)
- Delivery Days Customer9
- Application Supplier NoteTested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.
- ApplicationsWestern Blot, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID944569
- Target nameS
- Target descriptionlarge envelope protein;middle envelope protein;small envelope protein
- Target synonymsHBVgp2, large envelope protein;middle envelope protein;small envelope protein
- HostRabbit
- IsotypeIgG
- Protein IDD2X4M3
- Protein NameLarge S protein
- Scientific DescriptionBoster Bio Anti-Hepatitis B Virus Antibody Picoband® catalog # A30379. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- ReactivityHuman, Virus
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203
References
- Zhu X, Xie C, Li YM, et al. TMEM2 inhibits hepatitis B virus infection in HepG2 and HepG2.2.15 cells by activating the JAK-STAT signaling pathway. Cell Death Dis. 2016,7(6):e2239. doi: 10.1038/cddis.2016.146Read this paper
- Hu XL, Zhou XP, Qian YL, et al. The presence and expression of the hepatitis B virus in human oocytes and embryos. Hum Reprod. 2011,26(7):1860-7. doi: 10.1093/humrep/der103Read this paper
- Chen EQ, Song XQ, Wang YL, et al. Construction of a highly-active, liver-specific transcriptional regulatory element through combination of the albumin promoter and α-fetoprotein enhancer. Plasmid. 2011,65(2):125-31. doi: 10.1016/j.plasmid.2010.11.006Read this paper
Datasheet
MSDS
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Product group Antibodies
Anti-Hepatitis B Virus Antibody Picoband(r)A30379-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Virus
TargetS
- SizePrice
