Anti-HER2 [C7b]

Absolute Antibody

Anti-HER2 [C7b]

7

C7b specifically recognises HER2 at a different epitope to that recognised by Herceptin. HER2 is a protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential compon

The fine specificity of this antibody was determined by ELISA. The ELISA was preformed using the VHH version of this antibody on HER2 fused to an fc fragment. Furthermore the ability of the antibody to bind to cells expressing HER2 was assessed. This was done by flow cytometry on cells overexpressing HER2 using the VHH version of this antibody (Even-Desrumeaux et al, 2012; pmid:22772166). To assess the reactivity of this antibody, a dot blot was preformed on a ErbB2/HER2 Fc Chimera Protein using the VHH version of the antibody. Further, both a cytotoxicity assay and an internalization assay were preformed using this antibody bound to a model therapeutic nanoparticle (Vorotnikov et al, 2020; pmid:33107540). C7b has also been used to enrich breast cancer cells (SKBr3) from a large excess of human peripheral blood mononuclear cells (Even-Desrumeaux 2012).

Functional Assay, Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry

ELISA; IHC; cytometric bead array; FC; WB

Research Use Only

Monoclonal

C7b

ERBB2

erb-b2 receptor tyrosine kinase 2

CD340; c-erb B2/neu protein; HER2; HER-2; HER-2/neu; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; MLN 19; NEU; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; NGL; proto-oncogene c-ErbB-2; proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog; VSCN2

Rabbit

IgG

Receptor tyrosine-protein kinase erbB-2

This chimeric rabbit antibody was made using the variable domain sequences of the original VHH format, for improved compatibility with existing reagents, assays and techniques.

Human

Human

A llama was immunized with the HER2-expressing ovarian cancer cell line SKOv3 and a sdAb phage display library was built and used to perform biopannings. The first round of selection was performed on a purified recombinant HER2 ectodomain fused to a human Fc portion (HER2-Fc) followed by a second round on an HER2-positive SKBr3 cell line. After selection, 188 clones were screened and 92 clones were found to be positive by ELISA on HER2 positive cells. Twenty clones were analyzed by sequencing, and all of them corresponded to a unique clone named C7b.

-20°C,2°C to 8°C

12352203