Anti-Hexon [9C12]

Absolute Antibody

Anti-Hexon [9C12]

7

The antibody binds hexon protein from Ad5 particles. In particular, it binds to HVRs 2, 5 and 7 of the hexon. Hexon is the major coat protein of adenovirus.

The antibody was able to neutralize Ad5. Cryo-EM reconstruction of the Ad5-9C12 complex showed that the capsid is coated by a meshwork of bivalently bound antibody molecules. The antibody bound to the hexon protein of Ad5 particles denatured as shown by western blot analysis (Varghese et al., 2004; PMID: 15507619).The crystal structure of scFv fragment of the antibody in complex with human adenovirus C5 hexon was determined (Schmid et al., 2018; PMID: 29386504). The specificity of the original format of the antibody was confirmed by ELISA analysis (Moyer et al., 2016; PMID: 26491163). Wild-type viruses were immunoprecipitated using the antibody (Myerset al., 2013; PMID: 23487468). The binding affinity of the Fab fragment to Adv5 hexon was measured by surface plasmon resonance (Kd= 1 nM) (Bottermann et al., 2016; PMID: 27881870). The antibody detected hexon on A549 cells by immunofluorescence analysis (Smith et al., 2008; PMID: 18448546).

Electron Microscopy, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, Neutralisation/Blocking, Other Application

WB; IP; Neutralizing; Blocking; cryo-EM; ELISA; SPR; Crystallization; IF

Research Use Only

Monoclonal

9C12

Rabbit

IgG

Hexon protein

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Virus

Human Adenovirus

The original antibody was generated by immunizing mice with Ad type 5 (Ad5).

-20°C,2°C to 8°C

12352203