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Immunohistochemical staining of human prostate shows moderate membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows moderate membranous positivity in glandular cells.
Immunohistochemical staining of human prostate shows moderate membranous positivity in glandular cells.

Anti-HFE Antibody

HPA053470
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHFE
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HFE
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3077
  • Target name
    HFE
  • Target description
    homeostatic iron regulator
  • Target synonyms
    HFE1, HH, HLA-H, MVCD7, TFQTL2, hereditary hemochromatosis protein, MHC class I-like protein HFE, hereditary hemochromatosis protein HLA-H, high Fe
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ30201
  • Protein Name
    Hereditary hemochromatosis protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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HL-60 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with _x000D_ HFE/Hemochromatosis Polyclonal Antibody(bs-12335R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
Bioss
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Figure 1. Western blot analysis of HFE using anti-HFE antibody (A00506-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: HEPG2 whole Cell lysates, Lane 3: A431 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HFE antigen affinity purified polyclonal antibody (Catalog # A00506-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HFE at approximately 40KD. The expected band size for HFE is at 40KD.
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Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Product group Antibodies
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Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
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