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Immunohistochemical staining of human placenta shows moderate to strong nuclear and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate to strong nuclear and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate to strong nuclear and cytoplasmic positivity in trophoblastic cells.

Anti-HIF3A Antibody

HPA041141
Atlas Antibodies
ApplicationsChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetHIF3A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-HIF3A Antibody
  • Delivery Days Customer
    4
  • Applications
    ChIP Chromatin ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64344
  • Target name
    HIF3A
  • Target description
    hypoxia inducible factor 3 subunit alpha
  • Target synonyms
    HIF-3A, HIF3-alpha-1, IPAS, MOP7, PASD7, bHLHe17, hypoxia-inducible factor 3-alpha, PAS domain-containing protein 7, basic-helix-loop-helix-PAS protein MOP7, class E basic helix-loop-helix protein 17, hypoxia inducible factor 3 alpha subunit, inhibitory PAS domain protein, member of PAS protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2N7
  • Protein Name
    Hypoxia-inducible factor 3-alpha
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of HIF3A using anti-HIF3A antibody (A03805-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human U2OS whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF3A antigen affinity purified polyclonal antibody (Catalog # A03805-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF3A at approximately 72KD. The expected band size for HIF3A is at 72KD.
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