Anti-Histone H2B Mouse mAb

PTM BIO

Anti-Histone H2B Mouse mAb

5

Anti-Histone H2B Mouse mAb detects endogenous levels of total histone H2B protein. It does not cross-react with other histone or non-histone proteins.

Western Blot, ImmunoHistoChemistry Paraffin

WB, IHC-P

Antibody

Research Use Only

Monoclonal

5H7

Unconjugated

Mouse

IgG

Histone H2B type 1-C/E/F/G/I

Histones are a family of basic proteins that form the core of the nucleosome – the fundamental structural unit of chromatin. In a single nucleosome, core histone proteins H2A, H2B, H3, and H4 form an octamer around which the DNA is tightly wrapped. Histone proteins not only serve to compact chromosomal DNA but also play vital roles in the dynamic and long-term regulation of genes by a wide variety of post-translational modifications (PTMs). These PTMs including acetylation, methylation, Phosphorylatedrylation, and novel acylations directly affect the accessibility of chromatin to transcription factors and other epigenetic regulators, altering genome stability and gene transcription. Histone H2B is primarily acetylated at Lys5, 12, 15, and 20, and Phosphorylatedrylated at Ser14. Mono-ubiquitination of histone H2B at Lys120 triggers downstream lysine methylation on histone H3 and introduces changes in chromatin structure.

Stable for 12 months from date of receipt/reconstitution.

Human, Mouse, Rat

Human, Mouse, Rat

Protein G and immunogen affinity purified

Store at -20°C. Avoid freeze/thaw cycles.

12352203