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Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3.3 G34W mutant or wild type, stained with anti-Histone H3.3 G34W clone RM263.
Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3.3 G34W mutant or wild type, stained with anti-Histone H3.3 G34W clone RM263.
Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3.3 G34W mutant or wild type, stained with anti-Histone H3.3 G34W clone RM263.

anti-Histone H3.3 G34W Mutant (human), Rabbit Monoclonal (RM263)

Research Use Only
REV-31-1145-00
RevMAb Biosciences
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetH3-3A
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Overview

  • Supplier
    RevMAb Biosciences
  • Product Name
    anti-Histone H3.3 G34W Mutant (human), Rabbit Monoclonal (RM263)
  • Delivery Days Customer
    5
  • Antibody Specificity
    This antibody reacts to the Histone H3.3 G34W mutant. No cross reactivity with wild type Histone H3.3.
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RM263
  • Formulation
    Liquid
  • Gene ID3020
  • Target name
    H3-3A
  • Target description
    H3.3 histone A
  • Target synonyms
    H3 histone family member 3A; H3 histone, family 3A; H3.3A; H3-3B; H3F3; H3F3A; histone H3.3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP84243
  • Protein Name
    Histone H3.3
  • Scientific Description
    Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone H3 has three main variants, H3.1 and H3.2, which are deposited in chromatin only during DNA replication and H3.3, which is replication independent and is found primarily in the regions of active transcription and heterochromatin. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone modifications are one form of epigenetic information that relate closely to gene regulation. Aberrant histone methylation caused by alteration in chromatin-modifying enzymes has long been implicated in cancers. Recently, recurrent histone mutations have been identified in multiple cancers and have been shown to impede histone methylation. All identified histone mutations (including H3K4M, H3K9M, H3K27M, H3K36M, and H3G34V/R/W) result in amino acid substitution at/near a lysine residue that is a target of methylation. - Recombinant Antibody. This antibody reacts to the Histone H3.3 G34W mutant. No cross reactivity with wild type Histone H3.3. Applications: WB, ELISA, IHC, ICC. Source: Rabbit. Liquid. 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Histone H3 has three main variants, H3.1 and H3.2, which are deposited in chromatin only during DNA replication and H3.3, which is replication independent and is found primarily in the regions of active transcription and heterochromatin. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone modifications are one form of epigenetic information that relate closely to gene regulation. Aberrant histone methylation caused by alteration in chromatin-modifying enzymes has long been implicated in cancers. Recently, recurrent histone mutations have been identified in multiple cancers and have been shown to impede histone methylation. All identified histone mutations (including H3K4M, H3K9M, H3K27M, H3K36M, and H3G34V/R/W) result in amino acid substitution at/near a lysine residue that is a target of methylation.
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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