Anti-HIV-1 GP120 [b12 (MT12)]

Absolute Antibody

Anti-HIV-1 GP120 [b12 (MT12)]

9

This antibody binds CD4 binding site of the HIV-1 GP120.

Binding affinity of the Fab antibody and the IgG1 version towards HIV-1 gp120 was determined using ELISA (US5804440). This antibody is a potent neutralizer of HIV strains IIIB and MN. The IgG1 version of b12 antibody showed 50% neutralization titers of less than 40ng/ml for both IIIB and MN strain in a p24 reporter assay. b12 can neutralize about 75% of clade B strains of HIV-1 (those most common in North America), but it neutralizes less than 50% of other strains of HIV-1 found worldwide (PMID: 7973652). This antibody has also shown promise in complete protection against HIV-1 in hu-PBL-SCID mouse model by passive immunization. The in vivo protective dose against viral challenge was found to be 4.5-7mg/kg (PMID: 7662189). This antibody is also reported to neutralize HXBc2 HIV-1 strain (PMID: 7518527).

ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

b12 (MT12)

Rabbit

IgG

Envelope glycoprotein gp160

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Virus

-20°C,2°C to 8°C

12352203