Anti-HIV-1 GP120 [PGT124]

Absolute Antibody

Anti-HIV-1 GP120 [PGT124]

9

This broadly neutralizing antibody is capable of neutralizing HIV-1 strains 92RW020, 94UG103, 92BR020, JR-CSF, IAVI C22, BG505 and SF162. ELISA was used to determine the EC50 value of the antibody (PMID:28883821). To characterize the exact binding of this antibody, ITC binding experiments were performed using the fab version of this antibody with gp120 monomer. Further PGT124 Fab in complex with BG505 SOSIP.664 gp140 produced in HEK 293S cells were analyzed by negative stain EM. Finally To identify the epitope of the fab version of this antibody, the antibody was co-crystallized in complex with a gp120 core containing a mini-V3 loop. This crystal was subsequently used for crystallography to determine the structure of the complex (Garces et al, 2014; pmid:25259921).

Functional Assay, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

PGT124

Rabbit

IgG

Envelope glycoprotein gp160

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

41116161