Anti-HIV-1/HIV-2 protease [1696]

Absolute Antibody

Anti-HIV-1/HIV-2 protease [1696]

9

1696 binds to HIV-PR of both HIV-1 and HIV-2 (IgG 1696: HIV-1 PR pH5.7 ~350 uM; pH7.4 ~100 nM - HIV-2 PR pH5.7 no measurable interaction; pH7.4 ~30 nM -- Fab 1696: HIV-1 PR pH5.7 ~410 nM; pH7.4 ~130 nM - HIV-2 PR pH5.7 no measureable interaction; pH7.4 ~4

1696 mAb can inhibit the catalytic activity of both HIV-1 and HIV-2 HIV-PR (1696 IgG HIV-1 PR IC50 ~0.6 nM; HIV-2 PR IC50 ~1.5 nM -- 1696 Fab HIV-1 PR IC50 ~1.1 nM; HIV-2 PR IC50 ~2.6 nM). Inhibition by 1696 is mediated by the destabilization of the of the active form of the active HIV-PR homodimer through binding to the N-terminus, which constitutes a large percentage of the interface between the monomers. The Fab fragment was generated by incubation of the antibody with pepsin. Binding constants of 1696 can be determined by ELISA. 1696 shows reactivity to the mature processed HIV-PR and not to the precursor by WB.

Western Blot, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

1696

Mouse

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Virus

-20°C,2°C to 8°C

12352203